Tris (hydroxymethyl)aminomethane, commonly called TRIS or Trizma, is often used as a buffer in biochemical studies. Its buffering range is pH 7 to 9, and Kb is 1.19 ×10^-6 for the aqueous reaction (HOCH2)3 CNH2 H2 O ⇌(HOCH2)3 CNH3^ OH^- a. What is the optimal pH for TRIS buffers? b. Calculate the ratio [T R I S] /[T R I S H^ ] at p H=7.00 and at p H=9.00 c. A buffer is prepared by diluting 50.0 g TRIS base and 65.0 g TRIS hydrochloride (written as TRISHCl) to a total volume of 2.0 L What is the
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