The authors created a useful collection of Rep protein variants with changes that alter the amino acid sequence, but these did not lead to clear improvements in how the virus worked. Instead, their ...

This study presents an important finding on the role of GATA4 in aging and OA-associated cartilage pathology. The evidence supporting the conclusions is compelling, with rigorous in vitro and in vivo ...

Researchers at the National University of Singapore (NUS) have developed a scalable, non-viral technology that efficiently ...

Further we employed the acoustic-fluorescence-activated sorter to screen transfection cells from initial cells. Fluorescent SGC-7901-GFP cells were sorted from nonfluorescent SGC-7901 cells with the ...

For transfection of siRNA, oligonucleotides were complexed with ... Fluorophores were sequentially excited at 405 nm (DAPI), 488 nm (GFP, Alexa Fluor 488), 543 nm (Alexa Fluor 555), 561 nm (Alexa ...

Herein, we examine the effect of transfection media additives ... Heparin-coated polyplexes offer significant increases (approximately 4-fold) in GFP expression compared to polyplexes prepared with ...

MTCH2 KO MEFs were co-transfected with mt-PA-GFP construct and a mitochondrial matrix targeted DsRed fluorescent protein to identify transfected cells. 24 h post transfection cells were seeded in ...

postmitotic cardiomyocytes 2 days after transfection with the CCND2-cardiomyocyte SMRTs, and the increase was accompanied by the upregulation of markers for cell-cycle activation and proliferation (eg ...

Fig. 1. Detection of different ratios of WT-HeLas and GFP+/mcherry+-HeLas with the VantaStar, using fluorescence matrix scans and the bottom optic Fig. 2. Linear relationship of percentage of ...

UBQ10-NLS-tdTomato (Emonet et al., 2021) was co-transfected and served as a control to mark the transfected protoplasts and determine the transfection efficiency. Percentage recovery of the GFP ...

The library is stably integrated into chromosome. Transfection of Cas9+gRNA can induce GFP expression. GFP-positive cells are sorted out and synthesized off-targets are PCR-amplified for deep ...