Remember, in the context of any transfection experiment, a reading of “zero” does not rule out that some transfection has occurred. It merely indicates that whatever transfection did occur produced a ...

Ms. Sittenfeld is the author of the forthcoming story collection “Show Don’t Tell” and several novels. This summer, I agreed to a literary experiment with Times Opinion: What is the ...

Objective (1) To optimise the conditions of transfecting the liposome-mediated eukaryotic expression plasmid pAAV-IRES-hrGFP to H9C2 cells; (2) To verify the optimised parameters by magnified cell ...

Determination of transfection circumstances before the ... of whole fluorescent protein in each cell. Multicolor BiFC experiment involves the combination of substitutional partners and the ...

The experiment is part of a series that could ... The scientists will study the fibroblasts to understand how microgravity impacts transfection, a process where DNA is added to differentiated ...

The conclusion from this experiment was that the increase in viral titre in ... and viral vector yields were recorded at various points in the process (two hours before transfection, during ...

However, this claim is supported by one single transfection experiment, not quantified, which shows a slight reduction in UBE2H expression compared to wild-type MAEA. This is substandard. This ...

When choosing transfection reagents, researchers must identify the cell type and culture conditions for their experiment. Rare cell cultures such as neurons or primary cells require reagents that ...

When a cell is able to take up free DNA or RNA, it is termed “competent”. Some cell types or microbial species, such as Bacillus subtilis, 11 are naturally competent and they can be induced to uptake ...

Notably, the co-transfection experiment of the TST-EGFP-GPI BY55 expression plasmid and pmCherry-N1 plasmid manifested a strongly co-positive character for the two fluorescence. This property permits ...