The authors created a useful collection of Rep protein variants with changes that alter the amino acid sequence, but these did not lead to clear improvements in how the virus worked. Instead, their ...

HEK 293T cells were maintained at 37 °C under an atmosphere of 5% CO 2 in Dulbeccó’s modified Eagles medium (DMEM; Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS; Thermo ...

Herein, live confocal microscopy was used to track plasmid DNA during transfection of Chinese hamster ovary (CHO) and human embryonic kidney (HEK) cells to map key cellular processes central to ...

PASMCs (passages 4–7) and HEK293 cells were maintained in an incubator under 95% air or 1% O 2 (hypoxia treatment for 48–72 hours) and 5% CO 2 at 37 °C. For small interfering RNA transfection ... with ...

Comparison of stable polyclonal HEK cells expressing UNC13A-TS ... of the Super PiggyBac Transposase Expressing plasmid (PB200PA-1) using Lipofectamine 3000, according to the manufacturer’s guidelines ...

All PUFFFIN components are encoded on a single modular plasmid, amenable to delivery by transfection, electroporation ... We delivered the PUFFFIN plasmid to HEK293 cells and generated stable ...

A wide range of host cell lines are ... process (two hours before transfection, during transfection, and four hours after transfection). For AAV, three different serotypes were studied (AAV-2, AAV-5, ...

This method is as effective as standard biolistic transfection but is more appropriate for use in small cells such a HEK293 cells when examining cellular ... In turn, complexes of plasmid DNA (pDNA) ...

HEK 293 cells are also particularly useful in various transfection experiments ... enabling these cells to yield recombinant proteins in plasmid vectors that contain SV40 promoters. Having said that, ...

Although CRISPR-Cas9 has revolutionized genome editing, especially in experiments involving cell ... When plasmid DNA is used, it is important to consider the quality of the DNA. Transfection ...