Histological sections 5-μm thick were obtained from each sample and stained using the following histochemical techniques: hematoxylin and eosin to evaluate tissue integrity; Picrosirius red without ...

Samples were stained with picrosirius red to quantify collagen volume fraction. Clinical data at baseline and at last follow-up were reviewed. Adverse cardiovascular outcomes included death, sustained ...

The special picrosirius red staining highlights the natural birefringence of collagen fibers when exposed to polarized light. The results from birefringence allow to evaluate the organization of the ...

There was established postnecrotic bridging fibrosis (Panel A, upper inset [picrosirius red stain]; the lower inset shows equivalent upper-inset detail with hematoxylin and eosin stain).

5). Arrowheads indicate CD3 + cells. Nuclei were counterstained with DAPI (blue). Scale bar: 25 μm. E. Picrosirius Red and periodic acid‐Schiff (PAS) stainings of kidney nephrectomy samples from ADTKD ...

The new methodology is based on digitized images of patient biopsies stained with a dye called picrosirius red (PSR) and imaged with polarized light. The researchers use the open-source software ...

Quantification of invasion (H&E), collagen (Picrosirius Red) and αSMA presented to the right of image panels. *** P 0.001, ** P 0.01, * P 0.05, NS Not Significant, ANOVA with Dunnett’s post hoc ...

Digital images of entire lung histological serial sections stained with picrosirius red and alcian blue or anti-CD68 antibody were analyzed using dedicated software to automatically quantify fibrosis, ...

Myofibril mechanics and intact cardiomyocyte relaxation were assessed ex vivo. Cardiac fibrosis was evaluated by picrosirius red staining and second harmonic generation microscopy of left ventricle ...

Hematoxylin-Eosin (HE), Masson’s Trichrome and Picrosirius Red Staining After the tissues of left atrial wall were obtained, it was immediately fixed with 4% paraformaldehyde at 4°C and then embedded ...

Picrosirius red staining was used to detect collagen according to a published protocol for cryosections. 36 Tissues for histological analyses were extracted from 6 mice in the WHC+placebo group, 6 ...