Paraffin-embedded LV tissue sections (4 μm) were stained with picrosirius red to determine interstitial fibrosis using Aperio ScanScope Console v.8.0.0.1058 (AperioTechnologies, Inc). Picrosirius red ...

The special picrosirius red staining highlights the natural birefringence of collagen fibers when exposed to polarized light. The results from birefringence allow to evaluate the organization of the ...

7 Picrosirius red staining was used for the assessment of collagen fibers in the vessels. Sections were stained for 1 hour in saturated picric acid containing 0.1% picrosirius red (Direct Red 80, ...