Small interfering RNAs were purchased from GenePharma. Cells were harvested 24 to 48 hours after the transfection. Transient transfections were performed with Lipofectamine RNAiMax. Chromatin ...

¹ This comes as T cells are resistant to widespread commercially available chemical transfection reagents, which have a safety profile for numerous mammalian cells, like the Lipofectamine ...

Toxicity of transfection reagents is of particular concern in TGE ... In this study, we compare PEI, Lipofectamine ® 2000 and TransIT-PRO ® for the transient expression of the enhanced green ...

After 24h, 48h and 96h of transfection, the efficiencies were examined under a fluorescence microscope. Briefly, under the scope magnified by 1000 times, 10 different fields are randomly selected, in ...

while stFNA changed cell viability only when carrying 4 times the amount of miR carried by Lipofectamine 2000 (Li S. et al., 2021). However, the transfection efficiency of nucleic acid transporters ...

Transfection is a powerful analytical tool enabling studies ... characterized by higher gene expression in in vivo comparing with non-modified SLN–DNA and Lipofectamine 2000-DNA. Such a modification ...

Photothermal transfection was carried out by irradiation of cells co-treated with Lipofectamine–plasmid DNA complexes and PEGylated gold nanorods (GNRs) using an NIR laser for 30 min and subsequent ...

After transformed with the transfection reagent lipofectamine 2000, AFM data suggested a significant increase in roughness and adhesion force but an apparent decrease in Young's modulus. Besides, MTT ...

For comparison, the commercial liposomal transfection agent Lipofectamine was used. The cells were incubated with these delivery systems, carrying either enhanced green fluorescent protein (eGFP ...

Together, these results indicate that in vivo cardiac transfection of functional miRNAs can be achieved by intracardiac injection of cationic lipid formulations. Lipofectamine RNAiMAX was chosen for ...