“This is what I wanted to know. What are these green lines for?” Susana Pineda said. Pineda lived much of her life in New York City and never learned to drive. Now that she lives in Altamonte ...

As a result of the variety of applications several variants form the original wild type green fluorescent protein (wtGFP) have been developed. Several of these variants have different excitation and ...

The local excitation approximation (LEA), a method for the calculation ... acrolein surrounded by 24 water molecules, and a model of a green fluorescent protein. For all studied systems, the results ...

This allows us to identify fluorophores by absorption and emission. More precisely, as each fluorophore has a unique excitation spectrum and a unique emission spectrum, each peaking at a specific ...

For FRET to happen the fluorescence emission spectrum of the donor has to overlap with the ... Images were acquired in (A) the GFP channel (excitation GFP/emission GFP) and (B) the mCherry (excitation ...

Figure 1. a) Excitation and emission spectrum of GFP (Source: Biotium). Image Credit: Analytik Jena US Figure 1. b) Excitation and emission spectrum of RFP (Source: Biotium). The mouse model which ...

Excitation-inhibition (E:I) imbalance is theorized as an important ... Control animals were injected with a control AAV8-hSyn-GFP virus (www.addgene.com). These viral suspensions were injected using a ...

For the measurement of pH dependence of the fluorescence spectra, we used 50 mM buffers in the pH range ... The total number of atoms in the initial green form of the S65T GFP protein was equal to ...

The composite structure of the short-pass interference filter and absorption filters exhibited a band-pass spectrum between 510 and 570 nm, which is close to green fluorescent protein (GFP) emission.

The excitation spectrum acts here as a proxy for the absorption spectrum ... For comparison, the enhanced Green Fluorescent Protein (eGFP) has a Stokes shift of about 25 nm. So, if an electrochromic ...

Fig.3 Graph to illustrate the filter requirements for single wavelength imaging of GFP using a CoolLED pE-100470nm Fig.4 shows the logarithmic plots of the spectra of an excitation filter and emission ...