(b) Immunoblot showing LKB1-dependent phosphorylation of BRSK kinase domain proteins. (c) Thermal denaturation curves of BRSK catalytic domain proteins in the presence or absence of 10 mM DTT. (d) ...

The scattering curve of an empty capillary was recorded using the same ... which can be attributed to lysozyme denaturation. The samples with higher protein content also undergo glass transition and ...

2020) with modern quantitative proteomics. Using this approach, we developed solvent proteome profiling (SPP), which is capable of establishing target engagement through analysis of SPP denaturation ...

Reductionist approaches focusing on single-enzyme thermal performance curves, for instance, indicate that breakdowns in animal performance at high temperatures cannot simply be ascribed to protein ...

This fluorescence dye is a naturally quenched dye and interacts with the hydrophobic core of proteins, which is visible following denaturation. The midpoint, or melt peak, of the melting curve ...

Each of the four curves shows an endothermic peak between 72 °C and 100 °C, which is typical for protein denaturation. A difference is seen in the heat stability and enthalpy of denaturation of the ...

The experimental data (…), the approximation curve (___) according to eq. (2). The ΔBTME(t) of BSA decayed monotonically (Figure 2). This dependence was approximated by an empirical equation: MWR ...

Let’s talk about the “duck curve,” shall we? Everyone who cares about solar energy should know about the duck curve. Plus, it’s fun to say. Duck curve. Duck curve. The long story is below ...

(B) Thermal denaturation curve measured at 222 nm: open circles, experimental data; line, fitted curve obtained using a two-state transition model. Characterization of ENH-c2b oligomeric. (A) ...

The thermal stability change is measured by generating a thermal-denaturation curve in the presence of a hydrophobic fluorescent dye, such as Sypro Orange. As the protein unfolds, the hydrophobic ...