“One limitation [of Cre-LoxP], the focus of this editorial, is the potential loss of fidelity of Cre recombinase expression especially in the context of modeling cancer in mice.” “It is ...

The resulting experimental mouse is hemizygous for Cre and homozygous for loxP (D). This is the necessary genotype required to conditionally knock out the target gene in the specific tissue. Laura is ...

Cre-loxP organisms are generally produced by separately ... They also demonstrated that this technology can be applied to newts and mice. While continuing their research on newt regeneration ...

Now the same group has used the Cre/LoxP system to generate mice lacking the Tm gene specifically in endothelial cells. Many of these mice survive gestation but develop flagrant thrombosis in ...

The Cre enzyme recognises the loxP sequence and removes the coding fragment with the Dicer gene. “The first mice, in which the Dicer gene could be switched off at any time, were received by me a ...

Because the Cre-loxP recombination event is invisible ... Moreover, the use of the new iSuRe-Cre mice permits the induction of multiple genetic deletions in the same cell. This important property ...

The Cre-Lox system is commonly used for this ... the potential founder mice carried the properly inserted loxP sites. Founder mice were produced and our genetic validation strategies were able ...

A new editorial paper has been published in Oncoscience, titled, "Be mindful of potential pitfalls when using the Cre-LoxP system in cancer research." In this editorial, researchers Piotr Czarnota ...

In this new editorial, researchers Piotr Czarnota and Jaroslaw Cisowski from Jagiellonian University discuss Cre-LoxP - a widely used system to conditionally modify gene expression in mouse models ...