B-cell acute lymphoblastic leukemia (B-ALL) is the most common cancer in children, accounting for around 85% of pediatric ...

Somatic hypermutation (SHM) of immunoglobulin variable (V) regions modulates antibody-antigen affinity is initiated by activation-induced cytidine deaminase (AID) on single-stranded DNA (ssDNA).

This valuable study focuses on defining how the HSP70 chaperone system utilizes J-domain proteins to regulate the heat shock response-associated transcription factor HSF1. Using a combination of ...

RNA was extracted using RNeasy Micro Kit (Qiagen) and concentration quantified on NanoDrop ... TLR7-induced and IFN-induced activation of SLE B cells. (A) Overview of experimental setup: B cells from ...

Investigator-initiated trial (IIT) to be led by pioneers of cell therapy in B-cell mediated autoimmune disease, the Schett/Mackensen group at Friedrich-Alexander University Erlangen-Nürnberg - - Trial ...

Furthermore, neutrophils directly promoted B cell activation and cell differentiation ... Serum BAFF levels were measured by ELISA using Human BAFF/BLyS/ TNFSF13B Quantikine ELISA Kit (R&D Systems, ...

T cell activation plays a key role in the immune response. Its activation, proliferation and differentiation necessitate dual stimulation signals. The initial signal is made up of a combination of ...

Collectively, our results reveal that NMII exerts a B-cell-intrinsic inhibition on BCR signaling by regulating B-cell membrane contraction and surface BCR clustering, which curtails the activation of ...

B cells were enriched using the EasySep human pan-B cell enrichment kit (STEMCELL). CMV-E12-Flag (DYKDDDDK tag ... But Not B1a Cells, Led to NF-κB Activation, Upregulation of C-MYC Expression, and ...

Quantitative PCR Analysis and RNA Sequencing Sample Preparation Activated splenic B cells were harvested after 72 hours activation using LPS and IL-4. Polyadenylated RNA was isolated using Dynabeads ...

This, together with normal early signaling and activation of B cells in vitro and in vivo points to a defect ... and CD21/CD35 (7E9), CD45.1 (A20), CD138 (281‐2), and c‐kit (2B8) from BioLegend. For ...