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Western blot detection methods each have their own advantages ... As well as this, there is the high cost involved as it requires a dark room for development and maintenance costs. On the other hand, ...
A classic laboratory technique, western blotting, has been used by researchers for decades, and it is nearly obsolete. "Biologists currently are stuck in a time warp—they basically use the same ...
The key to a successful Western blot is achieving a signal to noise ratio ... Standard blocking is done for 1 hour at room temperature with agitation, or overnight at 4°C with agitation.
no dark room, developer or X-Ray film is needed. Use of different fluorescent dyes allows one-step multiplex detection of several different proteins. Using One-Step Fluorescent Western Kits ...
The western blot may appear to be just another staple lab technique. However, there are some stories surrounding its invention that are definitely worth knowing. This might be news to some: scientists ...
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