The authors created a useful collection of Rep protein variants with changes that alter the amino acid sequence, but these did not lead to clear improvements in how the virus worked. Instead, their ...

Gene transfection can produce engineered EVs by loading specific cargos into EVs and modifying EV membrane proteins. Exogenous nucleic acids (e.g., DNA, mRNA, siRNA, and miRNA) are transferred to ...

AAVPureMfg produces high-purity AAV vectors with reduced plasmid cost, which holds tremendous clinical and commercial significance. Inherent limitations in triple transfection are elucidated.

For manufacturing, the company utilizes suspension cultures of HEK293 cells. Iwuchukwu elaborates, “These cells are transfected with either triple or dual plasmid transfection systems ...