However, when developing this into a "probiotic" system that could spread through the gut on "low-copy" plasmids, the Thomas lab found that they had to engineer the plasmid to have a higher number ...

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Restriction digestions were performed with 1 μg of plasmid DNA in a final volume of 50 μL, at 37 °C for 1 h, using high-fidelity restriction enzymes (NEB ... oligonucleotides were stored at −20 °C.

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DNA plasmid depends on proteins and enzymes that the host of successful replication and transcription provides. It often contains genes which code enzymes that are important in a host cell in various ...

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Thursday, BioNTech said it finished construction at its first proprietary plasmid DNA manufacturing facility in Marburg, Germany. Plasmids are a key building block for mRNA- and cell-based drugs ...