The special picrosirius red staining highlights the natural birefringence of collagen fibers when exposed to polarized light. The results from birefringence allow to evaluate the organization of the ...

Paraffin-embedded LV tissue sections (4 μm) were stained with picrosirius red to determine interstitial fibrosis using Aperio ScanScope Console v.8.0.0.1058 (AperioTechnologies, Inc). Picrosirius red ...

Histological sections 5-μm thick were obtained from each sample and stained using the following histochemical techniques: hematoxylin and eosin to evaluate tissue integrity; Picrosirius red without ...

There was established postnecrotic bridging fibrosis (Panel A, upper inset [picrosirius red stain]; the lower inset shows equivalent upper-inset detail with hematoxylin and eosin stain).

5). Arrowheads indicate CD3 + cells. Nuclei were counterstained with DAPI (blue). Scale bar: 25 μm. E. Picrosirius Red and periodic acid‐Schiff (PAS) stainings of kidney nephrectomy samples from ADTKD ...

The new methodology is based on digitized images of patient biopsies stained with a dye called picrosirius red (PSR) and imaged with polarized light. The researchers use the open-source software ...

Digital images of entire lung histological serial sections stained with picrosirius red and alcian blue or anti-CD68 antibody were analyzed using dedicated software to automatically quantify fibrosis, ...

Myofibril mechanics and intact cardiomyocyte relaxation were assessed ex vivo. Cardiac fibrosis was evaluated by picrosirius red staining and second harmonic generation microscopy of left ventricle ...

Hematoxylin-Eosin (HE), Masson’s Trichrome and Picrosirius Red Staining After the tissues of left atrial wall were obtained, it was immediately fixed with 4% paraformaldehyde at 4°C and then embedded ...