The aminopterin in the HAT medium stops nucleotide synthesis, while hypoxanthine and thymidine can be used by cells, such as B cells, carrying the HGPRT (hypoxanthine-guanine phosphoribosyl ...

Lesch-Nyhan syndrome is a rare X-linked genetic disorder caused by a deficiency in the enzyme hypoxanthine-guanine phosphoribosyltransferase (HGPRT). This enzyme is involved in purine salvage, and its ...