Ideally, gene expression studies by quantitative RT-PCR (qRT-PCR) should be performed using RNA from homogeneous cell populations. Frequently, this means performing qRT-PCR using picogram amounts of ...

The alternative text for this image may have been generated using AI. AS assays harboring all possible nucleotides at positions 1 to 5 were designed for 6 SNPs involved in the TP53 pathway (Table 1).

In a recent study published on the medRxiv* preprint server, a team of researchers developed a new allele-specific (AS) real-time quantitative polymerase chain reaction (RT-qPCR) to detect the severe ...

Progressively more qPCR assays have been developed in recent years in numerous fields of application. These assays are routinely validated using calibration curves, but essential validation per se ...

Recent developments in digital PCR technology allow for highly sensitive detection of rare events and discrimination of very minute genomic and transcriptomic changes. [jxfzsy/Getty Images] Polymerase ...

Real-time polymerase chain reaction reverse transcription (RT-qPCR) has been described as the gold standard for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection. While the ...

Daniel Blanchard (left) is a molecular biologist and geneticist by training who, before joining Thermo Fisher Scientific (MA, USA), spent many years in academia, working mainly on RNA biology, RNAi ...

Determining agreement between immunohistochemistry and RT-qPCR for standard biomarkers in breast cancer: Validation on GEICAM 9906 clinical trial. Background: Trastuzumab therapy is indicated to ...