It is important here to distinguish between the terms transfection and transformation. While the term transfection is used when the host cells are eukaryotic, the term transformation is used to denote ...

When a cell is able to take up free DNA or RNA, it is termed “competent”. Some cell types or microbial species, such as Bacillus subtilis, 11 are naturally competent and they can be induced to uptake ...

and viral transfection approaches, electroporation alone provides a reasonable certainty of success regardless of the target cell or organism. For example, chemical transformation and ...

Here we present a standard protocol for genetic transformation of the rodent malarial parasite Plasmodium berghei using the nonviral Nucleofector technology for transfection 4. The improvement ...