Frozen competent cell solution Make a solution of 20% ... as plating density negatively affects transformation efficiency. Ito, H., Fukuda, Y., Murata, K. & Kimura, A. Transformation of intact ...

The transformation efficiency of E ... to effectively transform plasmid DNA into various strains of competent E. coli cells, which are acquiescent to heat shock transformation (Figure 7).

When a cell is able to take up free DNA or RNA, it is termed “competent”. Some cell types or microbial species, such as Bacillus subtilis, 11 are naturally competent and they can be induced to uptake ...