The technique mentioned in this article is suitable for the complete automation of bacterial transformation and colony growth. However, the protocol can be easily tailored for use on a standalone ...

Timing the culture of both the bacteria and plant cells such that both reach their logarithmic growth phase for the transformation step is essential to the success of the protocol. To achieve that ...

Bacteria, yeast, and plant protoplasts ... resulted in increased transformation efficiency. Another yeast transformation protocol calls for the use of LiAc/SS, as well as heat shock.

Throughout this protocol, S. epidermidis TÜ3298 wild ... e.g., in case of temperature-sensitive plasmids). Calculate the transformation efficiency as the number of transformants recovered per ...

A team has now introduced a novel approach that can make many more bacteria amenable to genetic engineering. Their method, called IMPRINT, uses cell-free systems to enhance DNA transformation ...

Let’s consider an example of a bacterial transformation protocol using chemically competent cells and heat shock. Both transfection and transformation are used to introduce foreign DNA into cells; ...