16 In this study, we have explored the use of lipofection and electroporation by nucleofection to enhance transfection of the genes for green fluorescent protein (GFP) and PEDF in ARPE-19 cells ...

Transfection, a key genetic technology in the ... The team made expression plasmids that fused green fluorescent protein (GFP) to the target membrane proteins and transfected the construct ...

The authors created a useful collection of Rep protein variants with changes that alter the amino acid sequence, but these did not lead to clear improvements in how the virus worked. Instead, their ...

Lastly, the transfection reaction itself was optimized ... Additionally, the binding of an anti-GFP antibody for its target protein in an immunoblot was demonstrated to be equivalent for antibody ...

You can use RT-PCR, western blot, FACS or imaging to evaluate transfection efficiency. My favorite validation method is to transfect with GFP plasmid or fluorescent control siRNA and quantify the ...

The most recent and sophisticated multi-component transfection reagent, FuGENE ® 4K, was created to transfer DNA into both difficult and routine mammalian cell lines. Users are able to obtain ...

In this interview, News Med talks to Hinnah Campwala about the pivotal role of transfection in modern research and drug discovery. Transfection is a tool that sees widespread use across a diverse ...

The flow cytometry and gene sequencing results showed that the cell viability of mesenchymal stem cells and primary T cells post-transfection remained at approximately 83.9% and 84.4% ...