Credit: Technology Networks. A) Before transfection: Plasmid DNA: The quality of plasmid DNA is very important for efficient transfection. The gene of interest is usually cloned into an appropriate ...

Cytochemical β-galactosidase staining after direct plasmid injection into the tumor and intravenous injection was performed. To investigate the in vivo transfection capabilities of ultrasound ...

Contamination with bacteria, yeast, virus, or mycoplasma will not only impair cellular health but also transfection efficiencies. The plasmid DNA concentration needs to be sufficient to obtain ...

The EGFP-PC3.1 plasmid was prediluted in Opti-MEM (1 µg/100 µL). The transfection-complex was formed by pipetting the same amount of plasmid dilution into the dilution of transfection reagent ...

Researchers deliver transfected nucleic acids as oligonucleotides or in a viral or plasmid vector, which carries the genetic material into the host cells. Transfection can be stable or transient.

Here, as part of our In Focus on transfection, Hinnah discusses transfection techniques, comparing the various nucleic acid selections and approaches available, and providing tips and tricks for best ...

Thermo Fisher Scientific has introduced the Applied Biosystems™ MagMAX™ Pro HT NoSpin Plasmid MiniPrep Kit to meet the ...

In this interview, News Med talks to Hinnah Campwala about the pivotal role of transfection in modern research and drug discovery. Transfection is a tool that sees widespread use across a diverse ...

If you work in a lab, whether you are researching the cutting edge of cell and gene therapy development or running a simple molecular binding screen, the chances are you have done some transfection in ...

The deal gives Sartorius additional know-how in nucleic acid delivery, including transfection reagents and plasmid DNA design, all of which are key elements in the production of viral vectors for ...