Credit: Technology Networks. A) Before transfection: Plasmid DNA: The quality of plasmid DNA is very important for efficient transfection. The gene of interest is usually cloned into an appropriate ...

Cytochemical β-galactosidase staining after direct plasmid injection into the tumor and intravenous injection was performed. To investigate the in vivo transfection capabilities of ultrasound ...

Contamination with bacteria, yeast, virus, or mycoplasma will not only impair cellular health but also transfection efficiencies. The plasmid DNA concentration needs to be sufficient to obtain ...

There are many barriers to transfection at a cellular level including the plasma membrane, the endosome membrane, and nuclear trafficking and entry (for plasmid DNA). According to Laura Juckem ...

Here, as part of our In Focus on transfection, Hinnah discusses transfection techniques, comparing the various nucleic acid selections and approaches available, and providing tips and tricks for best ...

Researchers deliver transfected nucleic acids as oligonucleotides or in a viral or plasmid vector, which carries the genetic material into the host cells. Transfection can be stable or transient.

Thermo Fisher Scientific has introduced the Applied Biosystems™ MagMAX™ Pro HT NoSpin Plasmid MiniPrep Kit to meet the ...

If you work in a lab, whether you are researching the cutting edge of cell and gene therapy development or running a simple molecular binding screen, the chances are you have done some transfection in ...

The most recent and sophisticated multi-component transfection reagent, FuGENE ® 4K, was created to transfer DNA into both difficult and routine mammalian cell lines. Users are able to obtain ...

The deal gives Sartorius additional know-how in nucleic acid delivery, including transfection reagents and plasmid DNA design, all of which are key elements in the production of viral vectors for ...