Transfection using the TransIT-PRO Transfection ... Cellular health is an important consideration prior to any tissue culture experiment. Ideally, cells should have a consistent doubling time ...

In this experiment, optimal conditions for HEK293 transfection were 5 µL of a 2.5:1 mix. Controls should be included in optimization experiments. Untransfected cells are used as an indication of ...

Here, as part of our In Focus on transfection, Hinnah discusses transfection techniques, comparing the various nucleic acid selections and approaches available, and providing tips and tricks for best ...

When choosing transfection reagents, researchers must identify the cell type and culture conditions for their experiment. Rare cell cultures such as neurons or primary cells require reagents that ...

"We also used another fluorescent protein, TagBFP, to gauge the variation in the transient transfection experiment," Wang adds. "In order to validate whether this experimental setup is suitable ...

Transfection is the process of deliberately ... types of plasmids into hard-to-transfect cells with high efficiency. Experiment results showed that dual types of plasmids could be delivered ...

Passage the cells before they reach ~90% confluency. This applies to any biology experiment, but is particularly important for siRNA transfection experiments. Do transfections with control siRNA ...

In this interview, News Med talks to Hinnah Campwala about the pivotal role of transfection in modern research and drug discovery. Transfection is a tool that sees widespread use across a diverse ...

DNA and mRNA transfection are both used to introduce genetic material into cells, but they differ in the genetic material used and cellular processes initiated. This infographic, part of our In Focus ...