Credit: Technology Networks. A) Before transfection: Plasmid DNA: The quality of plasmid DNA is very important for efficient transfection. The gene of interest is usually cloned into an appropriate ...

This study shows that sinusoidal focused ultrasound can significantly enhance the transfection of plasmid DNA in the Dunning prostate tumor R3327-AT1 in vitro and in vivo after direct plasmid ...

Contamination with bacteria, yeast, virus, or mycoplasma will not only impair cellular health but also transfection efficiencies. The plasmid DNA concentration needs to be sufficient to obtain ...

There are many barriers to transfection at a cellular level including the plasma membrane, the endosome membrane, and nuclear trafficking and entry (for plasmid DNA). According to Laura Juckem ...

Thermo Fisher Scientific has introduced the Applied Biosystems™ MagMAX™ Pro HT NoSpin Plasmid MiniPrep Kit to meet the ...

Analytik Jena‘s CyBio FeliX Liquid Handler facilitates the automatic isolation of transfection-quality plasmid DNA by way of applying a protocol for Promega‘s Wizard MagneSil Tfx ™ custom ...

Simply put, transfection is a foundational technique by which foreign nucleic acids such as DNA or RNA, can be introduced into mammalian host cells through non-viral means (e.g., liposomes, calcium ...

Researchers deliver transfected nucleic acids as oligonucleotides or in a viral or plasmid vector, which carries the genetic material into the host cells. Transfection can be stable or transient.

The two new products build on Roche’s 20 years of experience with transfection reagents ... incubate it with plasmid DNA, and pipette it directly onto cells. The simple process enables ...

As one of Amerigo Scientific's flagship transfection kits, the simple and rapid LipoMag kit has been widely used in all types of nucleic acids, including plasmid DNA, linearized DNA, siRNA ...