Ribonuclease (RNase) protection is a common and powerful technique used for the analysis of RNA levels. RNA probes used in RNase protection are commonly produced by in vitro transcription of DNA ...

This is a preview. Log in through your library . Abstract Polymerase chain reaction (PCR) was used to produce biotinylated DNA probes for human papillomavirus (HPV) types 16 and 18. The specificity ...

Scorpions ™ Primers integrate primer and probe into a single bifunctional molecule. Unlike dual-labeled probes and Molecular Beacons, Scorpions Primers combine primer and probe in one molecule, with ...

Real-time PCR or quantitative PCR (Polymerase Chain Reaction) is an advancement of the standard PCR technique, which enables continuous monitoring of the reaction and collection of data in real time.

PCR genetic analysis has been in the spotlight since COVID-19, but light is now further facilitating PCR-free methods. Osaka Metropolitan University scientists have developed a light-induced DNA ...

New probes using incredibly bright dyes could help detect COVID-19 earlier, leading to faster testing even when only trace amounts of SARS-CoV-2 RNA are present. As the COVID-19 pandemic continues, ...

In its formative years PCR and its intellectual possessors had to go through some legal wrangling over the originality of the technique. In point of fact, a 13-year earlier paper by Nobelist Gobind ...

High-resolution melting has proven to be a highly sensitive method for mutation discovery. PCR is performed in the presence of the double-strand DNA binding dye LCGreen® Plus. Samples are then ...

Human-rodent hybrid cell lines have been analyzed with regard to their human DNA content by using various DNA probe sets, derived from the hybrids, for in situ hybridization to normal human metaphase ...

As SARS-C0V-2 variants emerged, researchers developed a PCR test that uses molecular beacons not only to diagnose COVID-19 infection, but also to identify the specific variant causing that infection.

The earliest batch of COVID-19 tests distributed by the US Centers for Disease Control and Prevention (CDC) exhibited false positive reactivity of negative controls due to flaws in assay design and ...