We also found that homologous recombination is a prominent feature of the plasmid backbone evolution. Analysis of genomic signatures indicates that the plasmids have adapted to different host ...

By the same token, it is also important to use a host defective in RecA-mediated homologous recombination to prevent plasmid multimer formation by host recombination pathways. However, it has been ...

The DNA assembly strategy uses a proprietary enzyme mix based on the “chew-back and repair” principle exploited by homologous recombination. Up to four DNA fragments can be simultaneously ...

Selection of host strain also has a strong influence on the quality and quantity of plasmid produced. Host strains are generally K-12 derived and should be recA- to avoid homologous recombination ...

A unique feature of HR is that it repairs the damage to the DNA in a highly accurate way. In eukaryotic cells (those with a ...

If the inserted DNA is in the form of a plasmid then it is generally ... of the prophage into the host genome but rather by homologous recombination of the original host’s gal or bio genes ...

Homologous recombination plays essential roles in the repair of DNA double strand breaks (DSBs) and collapsed replication forks. The fundamental reaction in homologous recombination is the exchange of ...

Unlike its DNA repair role in somatic cells, homologous recombination has a distinct role in meiosis in ensuring proper chromosome segregation. In yeast, an early step of the meiotic program is the ...

New research shows bacteria form distinct species and maintain cohesion through frequent DNA exchange via homologous recombination. When Kostas Konstantinidis proved that many microbes -- like ...