fluorescence to monitor the protein conformational change associated with protein unfolding (denaturation). As illustrated in Figure 2, protein stability curves are generated by monitoring ...

The thermal stability change is measured by generating a thermal-denaturation curve in the presence of a hydrophobic fluorescent dye, such as Sypro Orange. As the protein unfolds, the hydrophobic ...

This fluorescence dye is a naturally quenched dye and interacts with the hydrophobic core of proteins, which is visible following denaturation. The midpoint, or melt peak, of the melting curve ...

Each of the four curves shows an endothermic peak between 72 °C and 100 °C, which is typical for protein denaturation. A difference is seen in the heat stability and enthalpy of denaturation of the ...