A new editorial paper has been published in Oncoscience, titled, "Be mindful of potential pitfalls when using the Cre-LoxP system in cancer research." In this editorial, researchers Piotr Czarnota ...

Cre-loxP organisms are generally produced by separately ... They also demonstrated that this technology can be applied to newts and mice. While continuing their research on newt regeneration ...

The resulting experimental mouse is hemizygous for Cre and homozygous for loxP (D). This is the necessary genotype required to conditionally knock out the target gene in the specific tissue. Laura is ...

The Cre enzyme recognises the loxP sequence and removes the coding fragment with the Dicer gene. “The first mice, in which the Dicer gene could be switched off at any time, were received by me a ...

The enzyme Cre (cyclization recombination) recombinase targets and cuts a DNA sequence and catalyzes recombination with another DNA locus (loxP). When a cell containing loxP sites in its genome ...

In mice, for example, the use of Cre recombinase to splice out specific genes flanked by loxP DNA sequences, has been used with some success. However, this methodology requires crossing the ...

the enzyme recognized the loxP sites and excised the STOP codon. Thus, active GFP expression served as an indicator of successful Cre delivery into the cells. But the true challenge lay ahead: to use ...

In this new editorial, researchers Piotr Czarnota and Jaroslaw Cisowski from Jagiellonian University discuss Cre-LoxP - a widely used system to conditionally modify gene expression in mouse models ...

Organisms modified via the introduction of the Cre-loxP system (Cre-loxP organisms) are widely used in life sciences to investigate the function of specific genes.Cre is a type of DNA ...