It is also a preferred method for CRISPR genome editing and for introducing complex genetic plasmids that enable the co-expression of multiple genes. Essentially, DNA transfection is a versatile ...

Recombinant baculovirus stocks from the transfection or co-transfection are plaque purified by an agarose overlay assay. Individual recombinant viral isolates are plucked from agarose and used to ...

The best way to regulate the variables introduced in transfection-based experiments ... control reporter vector is used which is then co-transfected with the empirical vector.

Academic research trends continue to drive transfection technology advancements. Researchers hope to use more biologically relevant yet hard-to-transfect cell models in addition to the standard ...

Transfection can be stable or transient. Stable transfection results in sustained, long-term expression, whereas expression will eventually be lost after transient transfection as host cells replicate ...

This can be challenging when using chemical transfection reagents,“ she explains. “Also, viral approaches can have their limitations, be it in insert size, co-transduction capabilities ...

The flow cytometry and gene sequencing results showed that the cell viability of mesenchymal stem cells and primary T cells post-transfection remained at approximately 83.9% and 84.4% ...