This step is particularly important, as too much or too little annexin V–FITC may give sub-optimal separation between healthy and apoptotic cell populations in the binding assays. PI staining.

2A2 and Fig. 2B2). Annexin V-FITC Staining. FTC 238 and FTC 133 cells were treated with doxorubicin (0.5 µM or 1 µM) for 4, 12, or 24 hours. The influence of doxorubicin on apoptosis was studied ...

Annexin-V is a specific PS-binding protein that can be used to detect apoptotic cells. Apoptosis can be induced by UV-irradiation, as well as by numerous chemical treatments including the use of ...

A new study highlights the effectiveness of MDX-124, the first therapeutic drug to target annexin-A1, a protein which is overexpressed in several cancer types and promotes tumor progression.

The bone marrow–biopsy specimen in Panel C shows leukemic hairy cells that express the CD20 antigen (immunoperoxidase stain). Panel D shows leukemic hairy cells that are positive for annexin A1 ...

Additionally, MDX-124 is shown to significantly inhibit tumor growth in in vivo models of triple-negative breast and pancreatic cancer, indicating that annexin-A1-targeted therapy represents a ...

The microarrays produced were used in a blinded validation study to determine whether annexin I, PGP9.5, and 14-3-3 theta antigens previously found to be targets of autoantibodies in newly diagnosed ...

Additionally, MDX-124 is shown to significantly inhibit tumor growth in in vivo models of triple-negative breast and pancreatic cancer, indicating that annexin-A1-targeted therapy represents a ...