Additional issues arise from the methods used to culture stem cells, with the majority of protocols relying upon 2D surfaces, which don’t replicate the 3D microenvironment native to cells in the body.

Moreover, in a 3D culture variant, by using tissue-specific stem cells, the villi of the small intestine and liver tissue can be reconstituted from a single adult stem cell 14,15. The key ...

The Global 3D Cell Culture Market is expected to witness a double-digit growth rate by 2027. Some of the key factors driving ...

To facilitate 3D biology applications ... the standard procedures so that the organoids do not lose the stem cell population. Culture conditions are extremely complex. Passaging and freezing ...

Researchers typically create organoid models for other biological systems with pluripotent stem cells, but the Harvard team began with readily accessible myoblasts, which satellite cells differentiate ...

Conventional methods for 3D cell culture rely on human intervention across all steps of growth, including seeding stem cells, collecting aggregates, feeding differentiating cells long term, and ...

The extracellular vesicles from the 3D-grown stem cells also exhibited ... vesicles by three‐dimensional dynamic culture of human mesenchymal stem cells, Journal of Extracellular Vesicles ...

Lab-grown organs, also known as organoids, are progressing from lab research toward clinical application. This article ...

His models primarily involve the use of 3D cultures and microphysiological systems generated from multi-species induced pluripotent stem cells. Here Szilagyi, reviews the utility of 3D cell models, ...

Stem cell cultures are derived from embryonic ... This includes the incorporation of multiple cell types, 3D architectures, and dynamic microenvironments. Future research in cell culture will focus on ...

Researchers led by Maike Sander, scientific director of the Max Delbrück Center, have developed a vascularized organoid model ...